Bisabolane has been identified as a potential biosynthetic alternative to D2 diesel fuel. Researchers at JBEI have engineered S. Cerevisiae for the production of bisabolene, bisabolane’s immediate precursor, by the introduction of bisabolene synthase from A. Grandis. In order to produce large enough quantities of bisabolane for engine testing and to better understand the challenges that arise at larger production scales, JBEI and the Advanced Biofuels Process Demonstration Unit (ABPDU) have developed a fed-batch fermentation process for bisabolene production for eventual scale-up to production scale. Presented here are preliminary results from 1.8 L fed-batch fermentations conducted at ABPDU with a discussion of challenges in scaling up the process.