Dry matter loss (DML) occurs in high-moisture storage conditions; it remains unclear how storage conditions and degradation impact sugar release and fermentation inhibitor production during conversion. In collaboration with Idaho National Labs, two feedstocks, switchgrass and corn stover, were compared using compositional analysis, alkaline pretreatment, and enzymatic saccharification. Under the tested conditions, switchgrass with 10% and 20% DML and corn stover with 30% DML achieved higher sugar yields compared to samples before storage.