Biosynthesis of highly branched short and long-chain hydrocarbons would enable production of biofuels with desirable and tunable properties, including compression ignition fuels with low freezing points. Polyketide synthase (PKS) pathways are a promising route towards production of such compounds; engineering of PKS pathways favors a native host- this work therefore focuses on development of Streptomyces venezualae ATCC 10712 as a platform organism. While S. venezualae is well characterized for industrial production of antibiotics, currently available protocols for high density fermentation make use of rich media and high-purity dextrose. The viability of Streptomyces as a platform organism for large-scale cellulosic biofuel production is therefore currently unknown.

This study focuses on the development of protocols for high density fed-batch fermentation of S. venezualae with cellulosic sugar feed in minimal medium to evaluate the viability of this strain as a production organism for fuels and commodity chemicals.