Lygos developed a biological process for malonic acid production and provided ABPDU with fermentation parameters optimized at the bench scale. At the 300-L scale, we used LabVIEW VI to control external pumps and regulate the progress of fermentation. The successful scale-up of this fermentation pathway demonstrated the ability in replacing traditional petroleum-based malonic acid production process, which requires hazardous cyanide and chloroacetic acid.