From Fermentable Sugars to Value-Added Precursors
Fermentation is a vital unit operation in the biomass conversion process offering the potential for complete utilization of biomass into multiple bioproducts. Of special interest in biorefining are platform intermediate chemicals from fermentation that can be converted into numerous consumer and industrial products, including succinic acid and butanol.
At ABPDU our focus is on biological fermentation as it offers the most selectivity and specificity when it comes to producing a product or intermediate. Microorganisms and recombinant organisms are used to metabolize lignocellulosic sugars and other intermediates to form a wide array of alcohols, acids, and enzymes—precursors to biofuels, biochemicals, and biomaterials.
High Yields Depend on Extensive Process Development
While fermentation is a very well understood unit operation in first-generation biofuels and bioproducts, it is a relatively new process area in the conversion of lignocellulosic sugars. Achieving high yields and economic viability requires extensive process development.
We apply process intensification and integration concepts to the traditionally removed fermentation and product recovery steps. Our intricate fermentation systems integrate either into deconstruction or recovery to assess the viability and economics of the integrated processes at a relevant scale.
Concept Development and Scale Up
Some companies design their pathway and select the organism to obtain a particular molecule. Based on your specifications, we can develop the fermentation process to a point where it is stable and ready for scale up.
Process Optimization and Validation
If you have a completely optimized bench scale fermentation process, we can control and optimize the process and then demonstrate it at a larger scale.
Achieving End Product Specifications
Feedstock can represent >40% of all process costs in a biomass-to-bioproduct process. Therefore, it is critical to rapidly and efficiently metabolize sugars and other molecules from biomass.
In designing a successful fermentation process, the required end product or intermediate drives our decision-making. Analytical chemistry is an essential part of our fermentation process development, enabling, among other things:
- Tight control of process parameters such as temperature, pH balance, nutrients, etc.
- Optimum selection of microorganisms and fermentation methods
- Improved product concentration
- Control of inhibitory effects for better process integration
Fermentation Process Options
Simultaneous Sachcharification and Co-Fermentation
Consolidated Bio Processing
Related Papers and Publications
This study explores the viability of streptomyces venezualae as a platform organism for large-scale cellulosic biofuel production
Mixed feedstocks can help reduce the risk associated with feedstock availability for bio-based production of fuels and chemicals.
In collaboration with Muufri, a fed-batch process to express milk proteins through Pichia species was optimized and scaled to 3L
Successful scale-up of the fermentation pathway for Lygos’ bio-malonic acid production.
Post-consumer absorbent hygiene products can be economically converted to fermentable sugar intermediates, biofuels, and bio-based products
Biosynthetic bisabolene is a precursor to bisabolane – a potential renewable diesel fuel alternative.
Bisabolane has been identified as a potential biosynthetic alternative to D2 diesel fuel. Researchers at JBEI have engineered S. Cerevisiae for the production of bisabolene, bisabolane’s immediate precursor, by the introduction of bisabolene synthase from A. Grandis.
Fed-batch fermentations involve controlled feeding of a growth limiting nutrient to a batch culture enabling higher cell densities. Some classic fed-batch strategies employ pH, OUR (oxygen uptake rate), DO (Dissolved oxygen) etc, as control parameters, based on their indirect correlations with growth.
Scale-up of Thermophilic Ionic Liquid-tolerant Cellulase Cocktail for Lignocellulosic Biofuel Production
Ionic liquid pretreatment of lignocellulosic biomass shows great potential in effectively reducing the biomass recalcitrance with less enzyme requirement for saccharification of biomass into sugars. However, commercially available cellulase enzymes are sensitive to...