Fermentation

From fermentable sugars to value-added precursors.

At ABPDU, our focus is on biological fermentation, as it offers the most selectivity and specificity when it comes to producing a product or intermediate. We use microorganisms and recombinant organisms to metabolize lignocellulosic sugars and other intermediates to create precursors to biofuels and bioproducts.

High Yields Depend on Extensive Process Development

We apply process intensification and integration concepts to the traditionally removed fermentation and product recovery steps. Our intricate fermentation systems integrate either into deconstruction or recovery to assess the viability and economics of the integrated processes at a relevant scale.

Based on your unique situation, we can develop the fermentation process to a point where it is stable and ready for scale up. If you have a completely optimized bench scale fermentation process, we can control and optimize the process and then demonstrate it at a larger scale.

The Sartorius ambr® 250 system is a high throughput, automated bioreactor system for process development.

Achieving End Product Specifications

In designing a successful fermentation process, the required end product or intermediate drives our decision-making. Analytical chemistry is an essential part of our fermentation process development, enabling, among other things:

  • Tight control of process parameters such as temperature, pH balance, nutrients, etc.
  • Optimum selection of microorganisms and fermentation methods
  • Improved product concentration
  • Control of inhibitory effects for better process integration

Fermentation Process Options

Environments

Aerobic and anaerobic fermentations in batch, fed-batch, and continuous modes.

Simultaneous Sachcharification

In simultaneous saccharification (SSF), the fermenting microorganism simultaneously consumes the released sugars from enzymatic hydrolysis, avoiding product inhibition of enzymes and also decreasing the probability of contamination.

Co-Fermentation

There are substantially greater savings in capital and operating costs to be gained by applying a co-fermentation process in which the hexose and pentose sugars derived from both the cellulose and hemicellulose fractions are simultaneously fermented to bio-product in a single operation. Using genetically modified organisms or a community of organisms, this method metabolizes more than one molecule at the same time, e.g glucose-6 carbon, xylose-5 carbon, and lignin.

Simultaneous Sachcharification and Co-Fermentation

Enzymatic hydrolysis can be performed simultaneously with the co-fermentation of glucose and xylose in a process referred to as simultaneous saccharification and co-fermentation (SScF). Besides reduced capital cost, the SScF process offers several advantages, which include continuous removal of end-products of enzymatic hydrolysis that inhibit cellulases or ╬▓-glucosidases and higher ethanol productivity and yield than separate hydrolysis and fermentation.

Consolidated Bio Processing

Consolidated bioprocessing (CBP) approaches combine enzyme production, substrate hydrolysis, and fermentation into one process. CBP has potential to lower the cost of biomass processing compared to process configurations featuring a dedicated step for cellulase production due to the elimination of operating and capital costs associated with dedicated enzyme production and more effective biomass solubilization.