Many Factors Impact Final Yield
Saccharification, the process to depolymerize cellulose and hemicellulose into fermentable sugars, is a considerable cost component in the biochemical conversion of biomass and feedstock to bioproducts.
In developing a high-yielding saccharification process, we take into consideration a combination of several factors:
- Biomass composition
- Type of pretreatment
- Dosage and efficiency of the hydrolytic catalyst or enzymes
Microbe Produces Ethanol from Switchgrass Without Pretreatment. Read article on science.energy.gov »
Integrating Upstream and Downstream Processes
Chemical pretreatment and saccharification are closely linked processes. While a particular pretreatment process might be effective against biomass recalcitrance, it may inhibit saccharification.
At ABPDU we place significant emphasis on the integration between pretreatment and saccharification to establish the optimum process parameters and selection of methodologies.
Saccharification Process Options
Cocktail Optimization of Enzymes
High Solids Enzymatic Hydrolysis
Simultaneous Saccharification and Fermentation (SSF)
Related Papers, Articles, and Presentations
Predictive modeling to de-risk bio-based manufacturing by adapting to variability in lignocellulosic biomass supply
This study develops a predictive model to optimize biomass blends for commercial-scale biorefinery processing
Mixed feedstocks can help reduce the risk associated with feedstock availability for bio-based production of fuels and chemicals.
Two case studies exploring conversion technologies for municipal solid waste. One is a partnership INL, and the other is a collaboration with FATER.
Switchgrass with 10% and 20% dry matter loss and corn stover with 30% dry matter loss achieved higher sugar yields during biomass conversion
Predictive modeling was used to evaluate and optimize traditional pretreatment methods for biomass mixture compositions to maximize sugar yield and minimize furfural production.
Municipal solid waste and corn stover blends present great potential to meet quality and cost requirements for sugar conversion.
The collaboration with INL and SNL demonstrated 600-fold (10mL to 6L) scale up of MSW/CS blends.
Post-consumer absorbent hygiene products can be economically converted to fermentable sugar intermediates, biofuels, and bio-based products
Municipal solid waste (MSW) represents an attractive cellulosic resource for sustainable fuel production. However, its heterogeneity is the major barrier to efficient conversion to biofuels. MSW paper mix was generated and blended with corn stover (CS) in an ionic liquid process.
Technologies developed to generate bio-based products are based on single feedstock types. While this approach is applicable for corn stover in the MidWest, for states such as California, with abundant but diverse feedstocks, technologies should be developed to accommodate multiple feedstock input to a single biorefinery.