Many Factors Impact Final Yield
Saccharification, the process to depolymerize cellulose and hemicellulose into fermentable sugars, is a considerable cost component in the biochemical conversion of biomass and feedstock to bioproducts.
In developing a high-yielding saccharification process, we take into consideration a combination of several factors:
- Biomass composition
- Type of pretreatment
- Dosage and efficiency of the hydrolytic catalyst or enzymes
Integrating Upstream and Downstream Processes
Chemical pretreatment and saccharification are closely linked processes. While a particular pretreatment process might be effective against biomass recalcitrance, it may inhibit saccharification.
At ABPDU we place significant emphasis on the integration between pretreatment and saccharification to establish the optimum process parameters and selection of methodologies.
Saccharification Process Options
Cocktail Optimization of Enzymes
High Solids Enzymatic Hydrolysis
Simultaneous Saccharification and Fermentation (SSF)
Related Papers, Articles, and Presentations
Ionic liquid pretreatment is receiving significant attention as a potential process that enables fractionation of lignocellulosic biomass and produces high yields of fermentable sugars suitable for the production of renewable fuels. However, successful optimization and scale up of ionic liquid pretreatment involves challenges, such as high solids loading, biomass handling and transfer, washing of pretreated solids and formation of inhibitors, which are not addressed during the development stages at the small scale in a laboratory environment. As a first in the research community, the Joint BioEnergy Institute, in collaboration with the Advanced Biofuels Process Demonstration Unit, a Department of Energy funded facility that supports academic and industrial entities in scaling their novel biofuels enabling technologies, have performed benchmark studies to identify key challenges associated with ionic liquid pretreatment using 1-ethyl-3-methylimidazolium acetate and subsequent enzymatic saccharification beyond bench scale.
Ionic liquid (IL) pretreatment has proven to be an effective method of biomass depolymerization for biofuel production. Understanding the physical and chemical properties of IL pretreated biomass at scale up level is essential to obtain better insights into challenges that may occur in large scale biorefineries. Building on the milliliter scale optimization, JBEI, in collaboration with Advanced Biofuels Process Demonstration Unit (ABPDU) is taking the first step to demonstrate IL pretreatment and subsequent saccharification at high solid loadings and liter scales (10 L), with a variety of feedstocks. Here, we provide the results of our studies aimed at understanding mass balances, residual ionic liquid inhibition of enzymes, and rheological properties of IL pretreated solids recovered from 10L scale.
To access the sugars in lignocellulosic biomass, pretreatment is an essential step to deconstruct the recalcitrant plant cell wall structures and facilitate enzymatic hydrolysis of recovered cellulose. Ionic liquid (IL) pretreatment is gaining substantial attention as a potential pretreatment process that can efficiently fractionate biomass and provide clean sugar substrate for the production of ethanol and other advanced biofuels. Previous work at Joint BioEnergy Institute (JBEI) has demonstrated at milliliter scales that IL can dissolve significant amounts of several feedstocks and produce highly digestible polysaccharides. However, a key factor in the development of economically viable lignocellulosic biofuels is to establish novel pretreatment technologies coupled with saccharification by advanced enzyme systems at process relevant scales.