Many Factors Impact Final Yield
Saccharification, the process to depolymerize cellulose and hemicellulose into fermentable sugars, is a considerable cost component in the biochemical conversion of biomass and feedstock to bioproducts.
In developing a high-yielding saccharification process, we take into consideration a combination of several factors:
- Biomass composition
- Type of pretreatment
- Dosage and efficiency of the hydrolytic catalyst or enzymes
Microbe Produces Ethanol from Switchgrass Without Pretreatment. Read article on science.energy.gov »
Integrating Upstream and Downstream Processes
Chemical pretreatment and saccharification are closely linked processes. While a particular pretreatment process might be effective against biomass recalcitrance, it may inhibit saccharification.
At ABPDU we place significant emphasis on the integration between pretreatment and saccharification to establish the optimum process parameters and selection of methodologies.
Saccharification Process Options
Cocktail Optimization of Enzymes
High Solids Enzymatic Hydrolysis
Simultaneous Saccharification and Fermentation (SSF)
Related Papers, Articles, and Presentations
The collaboration with INL and SNL demonstrated 600-fold (10mL to 6L) scale up of MSW/CS blends.
Post-consumer absorbent hygiene products can be economically converted to fermentable sugar intermediates, biofuels, and bio-based products
Municipal solid waste (MSW) represents an attractive cellulosic resource for sustainable fuel production. However, its heterogeneity is the major barrier to efficient conversion to biofuels. MSW paper mix was generated and blended with corn stover (CS) in an ionic liquid process.
Technologies developed to generate bio-based products are based on single feedstock types. While this approach is applicable for corn stover in the MidWest, for states such as California, with abundant but diverse feedstocks, technologies should be developed to accommodate multiple feedstock input to a single biorefinery.
Ionic liquid (IL) pretreatment is receiving significant attention as a potential process that enables fractionation of lignocellulosic biomass and produces high yields of fermentable sugars suitable for the production of renewable fuels.
Ionic liquid (IL) pretreatment has proven to be an effective method of biomass depolymerization for biofuel production. Understanding the physical and chemical properties of IL pretreated biomass at scale up level is essential to obtain better insights into challenges that may occur in large scale biorefineries.
To access the sugars in lignocellulosic biomass, pretreatment is an crucial step to deconstruct the recalcitrant plant cell wall structures and facilitate enzymatic hydrolysis of recovered cellulose.