Recovering the Most Value
In a biochemical conversion process producing advanced fuel or chemical molecules, the highest operating and equipment costs can often be found in the area of downstream processing—recovering and purifying the product from the broth after production. The purity of the product is mandated by market acceptance standards. The costs of the downstream processing steps, most often, increase with the standard of purity. Recovery yield and productivity also play a major role in final product cost.
Our ability to do different process configurations enables us to develop a high-performing, economically viable integrated process that is robust and scalable. Whether we’re extracting products according to your specifications, doing third party validation, or testing your concept, our broad depth of knowledge of the industry enables us to extract the most value from the starting material.
Unique Product Specifications Require Unique Process Parameters
Each product and intermediate is most likely required to have unique physical and chemical properties. The feed streams, the matrix, the reactions, and other process development influencers can further compound the uniqueness factor. In some cases, a single recovery step is not sufficient and will require the development of a recovery process chain.
Designed to Enhance Yield and Quality
At ABPDU, we define the different recovery and purification steps based on your final product specifications. In order to render the cost of the high-quality product more attractive, we explore any optimization–like the ones below– that could enhance recovery yield and product purity
- Each product can require different process parameters
- Each feedstock can lead to different separation and recovery processes
- Stepwise yields can be individually optimized
- Reagents can be substituted to decrease the cost factor
Purification & Advanced Recovery Process Options
Liquid-liquid solvent extraction (LLE)
Tangential flow filtration
Tangential flow filtration (TFF) is a rapid and efficient method for separation and purification of biomolecules. Your biomolecule of interest, or product, can be retained and separated from other low molecular weight contaminants, or it can be passed and purified from higher molecular weight contaminants and particles.
TFF can be used to concentrate and desalt sample solutions ranging in volume from 10 mL to thousands of liters. It can be used to fractionate large from small biomolecules, harvest cell suspensions, and clarify fermentation broths and cell lysates. At ABPDU, we have a continuous TFF at a feed flow rate of 4.5 mL/min that can separate biomolecules to up to 10 kDa.
Pilot-scale protein purification
Bulk freeze drying
Flow-through sonication and homogenization
Related Papers and Publications
In a collaboration with Muufri, a fed-batch process to express milk proteins through Pichia species was optimized by conducting several tests at 2L scale. The optimal process at 2L was scaled to 300L followed by centrifugation for supernatant recovery. The downstream recovery process was developed to concentrate and purify proteins from the supernatant. These proteins are now being tested in several milk formulations.
Utilization of biomass-based raw materials for the production of chemicals and materials is gaining an increasing interest. Due to the complex nature of biomass, a major challenge in its refining is the development of efficient fractionation and purification processes.
Preparative chromatography and membrane filtration are selective, energy-efficient separation techniques which offer a great potential for biorefinery applications. Both of these techniques have been widely studied. On the other hand, only few process concepts that combine the two methods have been presented in the literature. The aim of this thesis was to find the possible synergetic effects provided by combining chromatographic and membrane separations, with a particular interest in biorefinery separation processes. Such knowledge could be used in the development of new, more efficient separation processes for isolating valuable compounds from complex feed solutions that are typical for the biorefinery environment.